Enzymology

Daffodil International University Department of Genetic Engineering and Biotechnology Faculty of Health and Life Sciences B. Sc. (Hons.) in Genetic Engineering and Biotechnology Midterm Examination Fall 2026 Course Code: 0512-1307 Course Title: Enzymology Level and Term: L1, T3 Course Teacher Initials: DNH Time: 1 Hour 30 Minutes Total Marks: 25 Section: 261 (A) 1 (a) Define enzymology. Write the key features of enzymes. [CLO1, PLO1, C2] Marks: 3 (b) Briefly discuss the oxidoreductases and transferases enzymes with suitable examples [CLO1, PLO2, C2] Marks: 2 2 (a) State the approaches of enzyme nomenclature. Explain Enzyme Commission (EC) number of hexokinase (EC 2.7.1.1). [CLO1, PLO2, C6] Marks: 3 (b) Write the biological significance of enzyme specificity. [CLO1, PLO2, C6] Marks: 2 3 (a) Explain the induced-fit model of enzyme specificity with a suitable diagram, and discuss its limitations. [CLO2, PLO1, C4] Marks: 3 (b) Describe the different types of enzyme assays and explain their underlying principles. [CLO2, PLO2, C5] Marks: 2 4 (a) Write the factors affect the enzymatic reaction. How the temperature affects the enzymatic reaction? [CLO2, PLO1, C1] Marks: 3 (b) "At a certain point of enzymatic reaction, increment of substrate concentration do not increase the reaction rate". Explain the statement. [CLO2, PLO1, C2] Marks: 2 5 (a) Derive the Michaelis-Menten equation and explain the significance of Vmax and Km in enzyme kinetics. [CLO2, PLO1, C5] Marks: 4 (b) Identify the limitations of Lineweaver-Burk equation. [CLO3, PLO3, C3] Marks: 1